A multi modal clearing method for brain imaging

Year: 2015

Authors: Costantini I., Mascaro A.L.A., Di Giovanna A.P., Silvestri L., Müllenbroich M.C., Sacconi L., Pavone F.S.

Autors Affiliation: European Laboratory for Non-linear Spectroscopy, University of Florence, Via Nello Carrara 1, Sesto Fiorentino, 50019, Italy; National Institute of Optics, National Research Council, Largo Fermi 6, Florence, 50125, Italy; Department of Physics and Astronomy, University of Florence, Via Sansone 1, Sesto Fiorentino, 50019, Italy

Abstract: In this work we investigate the effectiveness of a water-soluble agent, the 2,2\’- thiodiethanol (TDE) to clear mouse and human brain. TDE is suitable with two-photon fluorescence microscopy and in combination with CLARITY with light sheet microscopy.

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KeyWords: Brain mapping; Fluorescence microscopy, Brain imaging; Human brain; Light-sheet microscopies; Multi-modal; Two-photon fluorescence microscopy; Water soluble agents, Neuroimaging
DOI: 10.1364/BRAIN.2015.BrM3B.2