Gravity driven high throughput phase detecting cytometer based on quantitative interferometric microscopy

Year: 2014

Authors: Xue L., Wang S., Yan K., Sun N., Ferraro P., Li Z., Liu F.

Autors Affiliation: Shanghai Univ Elect Power, Coll Elect & Informat Engn, Shanghai 200090, Peoples R China; Nanjing Univ Sci & Technol, Dept Informat Phys & Engn, Nanjing 210094, Jiangsu, Peoples R China; Xian Technol Univ, Sch Elect Informat Engn, Xian 710032, Shaanxi, Peoples R China; Shanghai Inst Qual Inspect & Tech Res, Inst Qual Inspect Elect & Elect Appliances, Shanghai 201114, Peoples R China; Ist Nazl Ottica Applicata CNR, I-80078 Pozzuoli, Napoli, Italy; Nanjing Agr Univ, Coll Vet Med, Single Mol Nanomehy Lab, Nanjing 210095, Jiangsu, Peoples R China.

Abstract: Phase distribution detection of cells and tissues is concerned since it is an important auxiliary method for observing biological samples. High speed and large amount cell detection is needed for its high detecting efficiency. In this paper, we have proposed a simple large scale biological sample phase detection device called gravity driven high throughput phase detecting cytometer based on quantitative interferometric microscopy to obtain flowing red blood cells phase. The system could realize high throughput phase detecting and statistical analysis with high detecting speed and in real time. The statistical characteristics of red blood cells could be obtained which might be helpful for biological analysis and disease detection. We believe this method is a powerful tool to quantitatively measure the phase distribution of biological samples. (C) 2013 Elsevier B.V. All rights reserved.

Journal/Review: OPTICS COMMUNICATIONS

Volume: 316      Pages from: 5  to: 9

More Information: This work was supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) for Fei Liu, the National Natural Science Foundation of China (Grant no. 31372399) and the Natural Science Foundation of Jiangsu Province (Grant no. BK20130699). The authors thank Yanke Shan in Nanjing Agricultural University for his kindly help in the experiments and Dr. Haijiao Jiang in Nanjing Institute of Astronomical Optics and Technology, Chinese Academy of Sciences for his kind help and discussions.
KeyWords: Interference microscopy; Phase retrieval; Medical optics instrumentation
DOI: 10.1016/j.optcom.2013.11.040

ImpactFactor: 1.449
Citations: 16
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