Wavefront-division lateral shearing autocorrelator for ultrafast laser microscopy

Year: 2004

Authors: Quercioli F., Tiribilli B., Vassalli M.

Autors Affiliation: Istituto Nzionale di Ottica Applicata, Biophotonics Laboratory, Largo E. Fermi 6, 50125 Firenze, Italy

Abstract: Nonlinear optical microscopy is a new and rapidly growing technique within which ultrafast laser technology finds a wide range of applications. Pulse widening, due to the microscope optics, is an issue of major concern for nonlinear excitation efficiency. We herewith describe a novel, simple and inexpensive autocorrelation technique to characterize the laser temporal behavior at the microscope focal plane. The method is based on a wavefront-division lateral shearing interferometer which is inserted into the microscope optical path like an ordinary filter, while a spatially uniform fluorescent specimen is observed. The two-photon excited fluorescent image provides the second-order autocorrelation curve. (C) 2004 Optical Society of America.

Journal/Review: OPTICS EXPRESS

Volume: 12 (18)      Pages from: 4303  to: 4312

KeyWords: Fluorescence; Imaging techniques; Interferometers; Multiphoton processes; Nonlinear optics; Optical correlation; Optical microscopy; Shearing, Fluorescence microscopy; Scanning microscopy; Ultrafast measurements; Wavefronts
DOI: 10.1364/OPEX.12.004303

ImpactFactor: 3.797
Citations: 11
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