Power-effective scanning with AODs for 3D optogenic applications
Year: 2022
Authors: Ricci P., Marchetti M., Sorelli M., Turrini L., Resta F., Gavryusev V., de Vito G., Sancataldo G., Vanzi F., Silvestri L., Pavone FS.
Autors Affiliation: European Lab Nonlinear Spect, I-50019 Florence, Italy; Univ Florence, Dept Phys & Astron, Florence, Italy; L4T Light4Tech, Florence, Italy; Univ Florence, Dept Neurosci Psychol Drug Res & Child Hlth, Florence, Italy; Univ Palermo, Dept Phys & Chem, Palermo, Italy; Univ Florence, Dept Biol, Florence, Italy; Natl Inst Opt, Florence, Italy.
Abstract: Two-photon (2P) excitation is a cornerstone approach widely employed in neuroscience microscopy for deep optical access and sub-micrometric-resolution light targeting into the brain. However, besides structural and functional imaging, 2P optogenetic stimulations are less routinary, especially in 3D. This is because of the adopted scanning systems, often feebly effective, slow and mechanically constricted. Faster illumination can be achieved through acousto-optic deflectors (AODs) although their applicability to large volumes excitation has been limited by large efficiency drop along the optical axis. Here, we present a new AOD-based scheme for 2P 3D scanning that improves the power delivery between different illumination planes. We applied this approach to photostimulate an optogenetic actuator in zebrafish larvae, demonstrating the method efficiency observing increased activity responses and uniform activation probabilities from neuronal clusters addressed in the volume. This novel driving scheme can open to new AOD applications in neuroscience, allowing more effective 3D interrogation in large neuronal networks.
Journal/Review: JOURNAL OF BIOPHOTONICS
Volume: 15 (4) Pages from: e202100256-1 to: e202100256-15
More Information: Open Access Funding provided by Universita degli Studi di Firenze within the CRUI-CARE Agreement.KeyWords: Access Multiphoton Microscopy; Neuronal-activity; 2-photon Excitation; High-speed; Optical Control; Interrogation; Indicators; RemoteDOI: 10.1002/jbio.202100256ImpactFactor: 2.800Citations: 3data from “WEB OF SCIENCE” (of Thomson Reuters) are update at: 2024-09-15References taken from IsiWeb of Knowledge: (subscribers only)
